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Recent Progress

Nature communications 10, 5688 (2019).

Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization.

Global genomic reorganization upon cellular senescence

In situ Hi-C experiments were performed using oncogene-induced senescence (OIS) and growing IMR90 cells. Gene contacts for chromosome 4 in OIS (top right) and growing cells (bottom left) are shown at 200 kb resolution. A global H3K9me3 pattern is shown on top. The in situ Hi-C data show that clustering of heterochromatin domains is stronger in OIS cells than proliferating cells. This means that heterochromatin aggregation visualized by DAPI staining (SAHF; right panel) can also be detected by the in situ Hi-C genomics approach.

Nature structural & molecular biology 24, 965–976 (2017).

Architectural alterations of the fission yeast genome during the cell cycle.

Intra-chromosomal (chr1) and inter-chromosomal (chr1 and chr2) contact maps during the fission yeast cell cycle

Fission yeast cells were cell cycle-synchronized using the cdc25-22 mutant and subjected to in situ Hi-C experiments, and the contact maps were generated throughout the cell cycle. Please note that the global gene contact patterns drastically alter during the cell cycle. The beautiful pattern showing genomic contacts mimics a pattern of maple leaf changing its color during autumn.

Annual Review of Genetics 51, 23-44 (2017)

The Yeast Genomes in Three Dimensions: Mechanisms and Functions

Three-dimensional genome organization and functions in nuclear events

Condensin and cohesin are loaded onto genomic regions by their recruiting factors. They mediate gene contacts and the formation of topological domains. The 3D genome structures organized by condensin and cohesin participate in chromosomal dynamics, transcriptional regulation, DNA repair, and other nuclear events.

Nature Genetics 48, 1242–1252 (2016).

Transcription factors mediate condensin recruitment and global chromosomal organization in fission yeast.

Genome-wide gene contacts mediated by condensin and cohesin

We employed the ChIA-PET genomic method to map condensin (left) and cohesin (right)-mediated gene contacts throughout the fission yeast genome. Blue and red lines represent gene contacts between two loci positioned more and less than 100 kb apart, respectively. Cen indicates centromeres.